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蛋白質組學

iTRAQ蛋白質組定量


背景簡介


        iTRAQ ( Isobaric Tags for Relative and Absolute Quantitation) 技術是ABI公司推出的一項新的體外同位素標記技術。使用該技術可以對一個基因組表達的全部蛋白質或一個復雜的混合體系中的所有蛋白質進行精確定量和鑒定。尋找差異表達蛋白,并揭示其細胞生理病理功能。聯川生物依托先進的蛋白質譜分析平臺、強大的信息分析和數據處理能力,為用戶提供高效的蛋白質組鑒定與定量分析服務。



 
 

技術優勢


通量高:一次實驗即可對數千種蛋白進行定量分析,且最多可同時對8份樣本進行定量分析

結果可靠:蛋白分析的靈敏度、特異性及重復性遠高于雙向電泳,并且定性與定量分析同步完成

覆蓋廣泛:鑒定與定量分析樣本中表達的所有已知蛋白,包括高分子量蛋白、酸堿性蛋白、膜蛋白和不溶性蛋白等

支持關聯分析:綜合上游轉錄組等實驗數據,提供多組學關聯分析




 


  

技術路線


 



 



分析內容


  




樣本類型


細胞,組織,尿液,全血,血清,血漿,總蛋白等

建議總蛋白起始量(單次):≥ 500 μg,濃度≥1μg/μL




 

用戶文章:

1.Wang L,et al. Proteome profiling reveals immune responses in Japanese flounder (Paralichthys olivaceus) infected with Edwardsiella tarda by iTRAQ analysis. Fish Shellfish Immunol. 2017 Jul;66:325-333. doi: 10.1016/j.fsi.2017.05.022

2.Zhang G, Zhang J, Wen X, et al. Comparative iTRAQ-based quantitative proteomic analysis of Pelteobagrus vachelli liver against acute hypoxia: Implications in metabolic responses.[J]. Proteomics, 2017.

3.Li R, Yu H, Yue Y, et al. Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish Cyanea nozakii.[J]. Journal of Proteomics, 2016, 148:57.

4.Lv LX, et al.(2016) Integrated transcriptomic and proteomic analysis of the bile stress response in probiotic Lactobacillus salivarius LI01. Journal of Proteomics. doi.org/10.1016/j.jprot.2016.08.021

5.Lv LX, et al.(2016) Integrated transcriptomic and proteomic analysis of the bile stress response in probiotic Lactobacillus salivarius LI01. Journal of Proteomics. doi.org/10.1016/j.jprot.2016.08.021

6.Wang C, Liu C, Wei L, Shi L, Pan Z, Mao L, Wan X, Ping Z, Jiang T, Chen Z. (2016) A Group of Novel Serum Diagnostic Biomarkers for Multidrug-Resistant Tuberculosis by iTRAQ-2D LC-MS/MS and Solexa Sequencing. International Journal of Biological Sciences 12(2), 246-256.

7.Cui Y, et al. (2016) Transcriptomic/proteomic identification of allergens in the mite Tyrophagus putrescentiae. Allergy.DOI:10.1111/all.12999

8.Cao JY, et al. (2016) TMT-based quantitative proteomics analyses reveal novel defense mechanisms of Brassica napus against the devastating necrotrophic pathogen Sclerotinia sclerotiorum. J Proteomics. doi: 10.1016/j.jprot

 

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