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轉錄組學

基因表達譜芯片


背景

        因表達譜分析是研究基因功能的基礎。利用基因表達譜芯片對不同發育時期或是不同處理條件下的樣品展開分析,可以廣泛快速可靠地篩選出差異表達基因,展開功能和通路分析。使用芯片進行基因表達譜分析已成為生理調控、生物標記、疾病機理和藥物篩選等領域廣泛采用的檢測手段。聯川生物為用戶提供基于穩定的Agilent芯片平臺的基因表達譜檢測。



 

技術優勢

探針特異性 & 靈敏度高:絕大多數探針都經過實驗驗證程序檢驗和優化,充分保證探針的特異性和靈敏度。

芯片檢測一致性高:具有極高的檢測重復性,充分保證檢測結果的準確性和一致性。

芯片更新靈活:探針序列設計基于RefSeq、Goldenpath、Ensembl和Unigene 等知名數據庫,充分代表基因和轉錄本信息。

芯片平臺可靠:芯片平臺均經過大量實驗數據的驗證,是分析基因表達譜高效可靠的工具。



 



技術路線




 



芯片信息



 


分析內容





樣本類型

細胞,組織,全血,血清,血漿,總RNA等

建議總RNA起始量(單次):≥ 1μg,濃度:≥50 ng/μL



近期用戶文章

1.tong Q, et al. (2016)  LincRNA-Cox2 modulates TNF-a–induced transcription of Il12b gene in intestinal epithelial cells through regulation of Mi-2/NuRD mediated epigenetic histone modifications. FASEB J, 30, 1187-1197 .

2. Guoku Hu,et al.(2016)LincRNA-Cox2 Promotes Late Inflammatory Gene Transcription in Macrophages through Modulating SWI/SNF-Mediated Chromatin Remodeling. The Journal of Immunology. 196 (6) :2799.

3. Feng J, Dai Z, Zhang Y, Meng L, Ye J, Ma X. (2015) Alteration of Gene Expression Profile in Kidney of Spontaneously Hypertensive Rats Treated with Protein Hydrolysate of Blue Mussel (Mytilus edulis) by DNA Microarray Analysis. PLoS ONE 10(10), e0142016.

4. Michael Zorniak, Paul A. Clark, John S. Kuo. (2014) Myelin-forming cell-specific cadherin-19 is a marker for minimally infiltrative glioblastoma stem-like cells. Journal of Neurosurgery. ePub ahead of print doi: 10.3171/2014.9.JNS132373.

5. Baulch JE, Aypar U, Waters KM, Yang AJ, Morgan WF. (2014) Genetic and Epigenetic Changes in Chromosomally Stable and Unstable Progeny of Irradiated Cells. PLoS ONE 9(9).

6. Kamal MM, Sathyan P, Singh SK, Zinn PO, Marisetty AL, Liang S, Gumin J, El-Mesallamy HO, Suki D, Colman H. (2012) REST regulates oncogenic properties of glioblastoma stem cells. Stem Cells 30(3), 405-414.

7. Thomas SN, Waters KM, Morgan WF, Yang AJ, Baulch JE. (2012)  Quantitative Proteomic Analysis of Mitochondrial Proteins Reveals Pro-Survival Mechanisms in the Perpetuation of Radiation Induced Genomic Instability. Free Radical Biology and Medicine 53(3), 618-28.


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